Dark-field modification
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georgetsmurf
- Posts: 46
- Joined: Tue Nov 30, 2010 5:55 pm
Dark-field modification
Sorry for my ignorance but new to this discipline. How does one set up a conventional microscope for Dark-field photomicrography?
This link explains and shows how it's done.
The two most common ways are using a darkfield condenser which uses an insert to block part of the light in a certain pattern. The other, cheaper, way is to cut some black card stock into a V shape and block some of the light from the immuminator below the stage. I have tried both. The condenser works best of course, but the cheap one has it's uses too.
The two most common ways are using a darkfield condenser which uses an insert to block part of the light in a certain pattern. The other, cheaper, way is to cut some black card stock into a V shape and block some of the light from the immuminator below the stage. I have tried both. The condenser works best of course, but the cheap one has it's uses too.
Last edited by Mitch640 on Mon Dec 06, 2010 3:56 pm, edited 1 time in total.
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Charles Krebs
- Posts: 5865
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- Location: Issaquah, WA USA
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While there are dedicated darkfield condensers, it's usually pretty easy to set up a standard condenser for use with objectives up to about 20X (or even a 40/0.65). All that is needed is an opaque disk (about the diameter of a dime) positioned under the condenser (as close to the condenser diaphragm as possible).
Does your microscope condenser have a "swing-out" filter tray under the condenser? If so, then that the place to put it, and it will be very easy to set up.
Does your microscope condenser have a "swing-out" filter tray under the condenser? If so, then that the place to put it, and it will be very easy to set up.