Spirogyra - Christmas spirit

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hkv
Posts: 478
Joined: Wed Jan 02, 2013 3:58 pm
Location: Sweden

Spirogyra - Christmas spirit

Post by hkv »

I want to wish you all a great holiday and Christmas by sharing this photograph of Spirogyra. Sort of like a Christmas gift wrapping or tree ornaments.

Spirogyra has one of the most fascinating chloroplast shapes of all algae – a helical shape, or spiral. Spirogyras thrive in almost any freshwater environment and are a common species that is easy to find in shallow ponds, ditches and lakes. For this photograph I stained some of the Spirogyra strands with a number of fluorescent dyes to highlight the spirally shaped chloroplasts. They were then mixed with natural strands and placed next to each other on a glass slide and photographed in fluorescent light.

Technical information:
Sony A9
Olympus UPlanSAPO 20x
ISO 250
0.3 sec
Accessories: Microscope Olympus BX51, SAPO 20X objective, fluorescent illumination.
Post processing: Zerene Stacker, Lightroom and Photoshop. Cropped, haze removal, noise reduction, contrast enhancement.

ImageSpirogyra_fluo_20x by Håkan Kvarnström Photography, on Flickr

Sumguy01
Posts: 1669
Joined: Mon Jan 28, 2013 11:05 pm
Location: Ketchikan Alaska USA

Re: Spirogyra - Christmas spirit

Post by Sumguy01 »

Very nice.
Thanks for sharing =D>
Happy Holidays too you also.

josmann
Posts: 12
Joined: Mon Nov 22, 2021 10:45 pm
Location: San Jose, CA

Re: Spirogyra - Christmas spirit

Post by josmann »

Cool microcomposition!

What were your excitation/emission parameters?
Last edited by josmann on Sun Dec 05, 2021 4:02 pm, edited 1 time in total.

hkv
Posts: 478
Joined: Wed Jan 02, 2013 3:58 pm
Location: Sweden

Re: Spirogyra - Christmas spirit

Post by hkv »

josmann wrote:
Sun Dec 05, 2021 11:46 am
Cool microcompositon!

What were your excitation/emission parameters?
Excitation Filter: 330 – 480 nm single band bandpass filter
Dichroic Mirror:495nm
Emission Filter: 500nm long pass edge filter

josmann
Posts: 12
Joined: Mon Nov 22, 2021 10:45 pm
Location: San Jose, CA

Re: Spirogyra - Christmas spirit

Post by josmann »

hkv wrote:
Sun Dec 05, 2021 1:12 pm
josmann wrote:
Sun Dec 05, 2021 11:46 am
Cool microcompositon!

What were your excitation/emission parameters?
Excitation Filter: 330 – 480 nm single band bandpass filter
Dichroic Mirror:495nm
Emission Filter: 500nm long pass edge filter
Oh wow, you're really blasting them with short wave! Is that a standard set or something you cooked up yourself?

hkv
Posts: 478
Joined: Wed Jan 02, 2013 3:58 pm
Location: Sweden

Re: Spirogyra - Christmas spirit

Post by hkv »

josmann wrote:
Sun Dec 05, 2021 4:09 pm
hkv wrote:
Sun Dec 05, 2021 1:12 pm
josmann wrote:
Sun Dec 05, 2021 11:46 am
Cool microcompositon!

What were your excitation/emission parameters?
Excitation Filter: 330 – 480 nm single band bandpass filter
Dichroic Mirror:495nm
Emission Filter: 500nm long pass edge filter
Oh wow, you're really blasting them with short wave! Is that a standard set or something you cooked up yourself?
I made it myself out of eBay surplus filters.

KurtM
Posts: 83
Joined: Wed Jul 16, 2014 12:20 pm
Location: League City, Texas
Contact:

Re: Spirogyra - Christmas spirit

Post by KurtM »

Very Christmassy alright! Cheers back at you!
Cheers,
Kurt Maurer
League City, Texas

ModelZ
Posts: 56
Joined: Sun Nov 01, 2020 4:34 am
Location: Northern Europe

Re: Spirogyra - Christmas spirit

Post by ModelZ »

Nice idea and rendering!
Spirogyras have a fair bit of variability. Tighter and more loosely wound, smoother and more convoluted ribbons like you have etc. Here all are single strands but even in the same location one can with some luck find double and triple strand types. Beautifully intertwined inside the tube - the separate strands rarely make a mess even with those bulges&folds in chlorophyl ribbon.

Bob-O-Rama
Posts: 115
Joined: Sat Dec 06, 2014 6:46 am
Location: Allentown, PA, USA, Earth, etc.
Contact:

Re: Spirogyra - Christmas spirit

Post by Bob-O-Rama »

That is a very festive and creative microscopic garland.

soldevilla
Posts: 637
Joined: Thu Dec 16, 2010 2:49 pm
Location: Barcelona, more or less

Re: Spirogyra - Christmas spirit

Post by soldevilla »

I want some Spirogyra for my Christmas tree =D> =D> =D>

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