Snail tongues
Moderators: rjlittlefield, ChrisR, Chris S., Pau
-
- Posts: 188
- Joined: Fri Nov 28, 2014 12:39 pm
- Location: Virginia, USA
- Contact:
Snail tongues
Radula – a rasping tongue – of an Astraea conehead snail, Astraea tecta, an apple snail, Ampullaria sp. and a zebra nerite snail, Neritina natalensis. Depth color-coded projections.
Staining: Congo Red.
Imaging: 10x/0.45NA objective; ZEISS LSM 880.
Staining: Congo Red.
Imaging: 10x/0.45NA objective; ZEISS LSM 880.
- rjlittlefield
- Site Admin
- Posts: 23606
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
Re: Snail tongues
These are lovely!
I think I understand that these images started off as fluorescent confocal, and the Congo Red stain is to provide the fluorescence.
But then I am intrigued that https://en.wikipedia.org/wiki/Congo_red does not mention anything about fluorescence.
Has Wikipedia missed something?!
--Rik
I think I understand that these images started off as fluorescent confocal, and the Congo Red stain is to provide the fluorescence.
But then I am intrigued that https://en.wikipedia.org/wiki/Congo_red does not mention anything about fluorescence.
Has Wikipedia missed something?!
--Rik
-
- Posts: 188
- Joined: Fri Nov 28, 2014 12:39 pm
- Location: Virginia, USA
- Contact:
Re: Snail tongues
Thanks, Rik!
Yes, apparently Wikipedia forgot to mention the fluorescence of Congo Red - which, by the way, is super bright and quite resistant to photo-bleaching. Pleasure to work with.
Yes, apparently Wikipedia forgot to mention the fluorescence of Congo Red - which, by the way, is super bright and quite resistant to photo-bleaching. Pleasure to work with.
rjlittlefield wrote: ↑Wed Mar 16, 2022 5:43 pmThese are lovely!
I think I understand that these images started off as fluorescent confocal, and the Congo Red stain is to provide the fluorescence.
But then I am intrigued that https://en.wikipedia.org/wiki/Congo_red does not mention anything about fluorescence.
Has Wikipedia missed something?!
--Rik
-
- Posts: 5090
- Joined: Sun Jan 15, 2012 12:31 pm
Re: Snail tongues
Those are crazy wonderful!
Mike
Mike
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
- rjlittlefield
- Site Admin
- Posts: 23606
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
Re: Snail tongues
Good news, the Wikipedia article now mentions "confocal" and "fluorescent".
I forget, is there any way to get these in stereo? If so, I would love to see at least one as stereo monochrome.
--Rik
I forget, is there any way to get these in stereo? If so, I would love to see at least one as stereo monochrome.
--Rik
-
- Posts: 1527
- Joined: Mon Jan 15, 2018 9:23 pm
- Contact:
Re: Snail tongues
These are beautiful, one day I will also own a confocal microscope and have the ability to prepare such slides.
-
- Posts: 188
- Joined: Fri Nov 28, 2014 12:39 pm
- Location: Virginia, USA
- Contact:
Re: Snail tongues
Hi Rik, I have some red-cyan anaglyphs, would those work?
rjlittlefield wrote: ↑Thu Mar 17, 2022 5:29 pmGood news, the Wikipedia article now mentions "confocal" and "fluorescent".
I forget, is there any way to get these in stereo? If so, I would love to see at least one as stereo monochrome.
--Rik
Re: Snail tongues
Wow, amazing! How did you clean them?
René
René
Re: Snail tongues
Superb! Almost graphic art rather than photos.
-Karl
-Karl
- rjlittlefield
- Site Admin
- Posts: 23606
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
Re: Snail tongues
Thanks for the stereo! That first one is spectacularly clean!
--Rik
--Rik
-
- Posts: 188
- Joined: Fri Nov 28, 2014 12:39 pm
- Location: Virginia, USA
- Contact:
- rjlittlefield
- Site Admin
- Posts: 23606
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
Re: Snail tongues
What concentration?
--Rik
-
- Posts: 188
- Joined: Fri Nov 28, 2014 12:39 pm
- Location: Virginia, USA
- Contact:
Re: Snail tongues
I don't really know, I eyeball the amount Likely around 5-10 mg/ml, in PBS.
Re: Snail tongues
Beautiful! Science as art.
When you say "depth color-coded", does that mean you could convert color into a depth-map and then do a horizontal displacement shift
in photoshop (or other) in order to get a full color side-by-side stereo view? Love to see that.
John
hart3d.com
When you say "depth color-coded", does that mean you could convert color into a depth-map and then do a horizontal displacement shift
in photoshop (or other) in order to get a full color side-by-side stereo view? Love to see that.
John
hart3d.com
-
- Posts: 188
- Joined: Fri Nov 28, 2014 12:39 pm
- Location: Virginia, USA
- Contact:
Re: Snail tongues
Thanks! Sometimes science is more art than science
I can easily convert a stack into depth color-coded stack (although only one color palette, or lookup table, is available in this mode), and make a stereo pair in ImageJ/Fiji (see below). To get a full 3d experience I extracted a mesh out of the confocal volume of one of the samples and rendered a fly-by video, but to see it you'd have to find me on Instagram Below are 2 frames from it.
I can easily convert a stack into depth color-coded stack (although only one color palette, or lookup table, is available in this mode), and make a stereo pair in ImageJ/Fiji (see below). To get a full 3d experience I extracted a mesh out of the confocal volume of one of the samples and rendered a fly-by video, but to see it you'd have to find me on Instagram Below are 2 frames from it.
hart3d wrote: ↑Sat Mar 19, 2022 7:13 amBeautiful! Science as art.
When you say "depth color-coded", does that mean you could convert color into a depth-map and then do a horizontal displacement shift
in photoshop (or other) in order to get a full color side-by-side stereo view? Love to see that.
John
hart3d.com