Polarized midge larvae

Images made through a microscope. All subject types.

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seta666
Posts: 1071
Joined: Fri Mar 19, 2010 8:50 am
Location: Castellon, Spain

Polarized midge larvae

Post by seta666 »

I did a whole series trying my polarizing set up, on top of the source light there is a 52mm polarizer, then in the middle of the swing out condenser a clear (or frosted) plastic CD protector from a CD box working as a retarder (kind of)and just below the camera the analyzer on a 30 to 37mm step up ring (makes it easy to remove it)

Image ImageImage

First one is the front part with Nikon CF 10/0.50 Fluor via Direct projection on a Olympus BHT, XPol + retarder, 64 image stack with Sony A5100

Image

Big size

Stereo Pair

One more, same setup different adjustments; 66 images

Image

Big size

Stereo pair

Another one similar to the first one, different polarizer settings; 56 shots

Image

Big size

Stereo pair
Last edited by seta666 on Fri Apr 09, 2021 12:22 pm, edited 7 times in total.

NikonUser
Posts: 2693
Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Re: Polarized mosquito (or midge) larvae

Post by NikonUser »

Excellent image; it's a Chironomid (midge)
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

seta666
Posts: 1071
Joined: Fri Mar 19, 2010 8:50 am
Location: Castellon, Spain

Re: Polarized mosquito (or midge) larvae

Post by seta666 »

NikonUser wrote:
Wed Apr 07, 2021 7:44 am
Excellent image; it's a Chironomid (midge)
Thanks, I am lucky I have you (and Rik ) to correct me all the time .. :D

micro_pix
Posts: 469
Joined: Fri May 11, 2012 12:05 pm
Location: Southampton, Hampshire, UK

Re: Polarized midge larvae

Post by micro_pix »

Great result! The stereo is excellent.

Dave

soldevilla
Posts: 684
Joined: Thu Dec 16, 2010 2:49 pm
Location: Barcelona, more or less

Re: Polarized midge larvae

Post by soldevilla »

Nice images! . You do not have loss of contrast when using a polarizer between the object and the objective? I have never achieved a nice result and with minerals with shiny surfaces it should work well.

seta666
Posts: 1071
Joined: Fri Mar 19, 2010 8:50 am
Location: Castellon, Spain

Re: Polarized midge larvae

Post by seta666 »

soldevilla wrote:
Thu Apr 08, 2021 11:04 am
Nice images! . You do not have loss of contrast when using a polarizer between the object and the objective? I have never achieved a nice result and with minerals with shiny surfaces it should work well.
Thanks!! It is not between object and objective; the polarizer is above source light, then in the middle of the condenser plastic from a CD box acts as a retarder, the the object, objective and analyzer just below the camera

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