Various

Images made through a microscope. All subject types.

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Jacek
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Joined: Sun Oct 02, 2011 7:00 am
Location: Poland

Various

Post by Jacek »

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Harald
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Location: Steinberg, Norway
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Post by Harald »

Hi Jacek,
Great set of images as always.
Love the 3rd one of the moquito, amazing details and light.

Thanks for sharing :D
Kind Regards
Harald

Lier Fotoklubb / NSFF
AFIAP / CPS
BGF / GMV
http://www.500px.com/blender11

MASZEK
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Location: POLAND

Post by MASZEK »

Hi Jacek.
My whole comment: great photos.
All. :D

anne
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Post by anne »

Absolutely stunning images!
Thank you for sharing.
anne

santiago
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Location: Nijmegen, The Netherlands

Post by santiago »

Amazing images!
Santiago
Flickr

Lou Jost
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Post by Lou Jost »

These are such beautiful photos. I only wish that you would always include some minimal information about the techniques and objectives/lenses for each of these inspiring images.

viktor j nilsson
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Location: Lund, Sweden

Post by viktor j nilsson »

Just... wow. I agree with Lou, I'd really appreciate at least a brief note about the objectives and illumination for these images. In the meantime, I drool.

Jacek
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Joined: Sun Oct 02, 2011 7:00 am
Location: Poland

Post by Jacek »

Thank you very much :)

EPI (two Ikea lights + diffuser)

1. EPI + Pol (dia) (Zeiss Apo 6.3/20)
2. Pol (Zeiss Apo 6.3/20)
3. EPI (Nikon CFN PlanApo 10/0.45)
4. Pol (Nikon CFN PlanApo 10/0.45)
5. autoFL (UV) (Nikon CFN PlanApo 40/0.95)
6. autoFL (UV) (Zeiss Apo 6.3/20)
7. EPI (Nikon CFN Plan 4/0.13)

Next set:

1. BF (Nikon CFN PlanApo 10/0.45)
2. BF (Zeiss Apo 6.3/20)
3. autoFL (UV) (Zeiss Apo 6.3/20)
4. EPI (Nikon CFN PlanApo 10/0.45)
5. EPI (Zeiss Apo 6.3/20)
6. DIC (Nikon CFN Plan Fluor 40/0.75)
7. DIC (Nikon CFN Plan Fluor 40/0.75)

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Lou Jost
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Post by Lou Jost »

Thanks very much for the added information and for the second set! It is much appreciated.

ChrisR
Site Admin
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Post by ChrisR »

Super pictures, again :)

I believe you are using afocal method, yes?
When you change from Zeiss to Nikon, do you change eyepieces?

I apologize if you have already covered this!
Chris R

Jacek
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Joined: Sun Oct 02, 2011 7:00 am
Location: Poland

Post by Jacek »

Yes, I'm still doing it afokal, I do not change my glasses.
I have Chinese glasses 10X/20 with a rejected pupil.
I use Nikon CFWN 10X/20 glasses only for DIC.

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pbraub
Posts: 60
Joined: Fri Feb 02, 2018 1:23 pm

Post by pbraub »

Spectacular images, thank you Jacek!

What lightsource do you use for UV excitation? Do you excite through the objective or from the side?

Kind regards
Peter

Robert Berdan
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Amazing Photomicrographs

Post by Robert Berdan »

Exceptional images Jacek you are an inspiration. Well done.
Rob

Sumguy01
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Location: Ketchikan Alaska USA

Post by Sumguy01 »

:smt038 =D> Excellent photos.
Thanks for sharing.

Jacek
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Joined: Sun Oct 02, 2011 7:00 am
Location: Poland

Post by Jacek »

Thanks :)

I use fluorescence on two microscopes. On one FL epi (LED lighting 3W 365 nm (UV) and 460 nm (blue)).
On the second one I have FL dia, one diode 365 nm on it, a converted condenser, enlarging the diode + UG5 filter and barrier filter.
In addition, for small magnifications (10X lens and small) I use two illuminators that I have made (365 LEDs + UG5 excitation filters) - this solution is very efficient lighting.

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