Norm, excellent image I have a Zeiss standard microscope and I'm interested in a DIC setup, but, apart of very expensive, I find it difficult to understand. I'm aware of two diferent sets from Zeiss, one with prims on each objective and another whit a common prism in the tube, and I think the conden...

In my limited knowledge, a good condenser adjustement is very important (Köhler if posible, critical if your microscope don't allow Köhler), and its importance increases whith the objective N.A. In fact, the resolution loss and uneven illumination issues are much more noticeable in photomicrography ...

ChrisR wrote: Many creatures seemed to be moribund, such as all these. Time of year?

Perhaps an insecticide fumigation?. I did see this sad scene many times near the orange tree fields here in Valencia

Mike, If the high magnification eyepiece is for the phototube, I do not recommend it: you will have higher magnification, of course, but no better resolution, and the image may be softer. In general, the best aproach is to switch to higher magnification objectives whith higher N.A. because resolutio...

Will, Very nice. I use the Mathias wedge in my Biology courses, but not tried it seriously for photomicrography. It's much easier to set up in my scholar microscopes (Olympus CH chinese clones whith Abbe condensers) than in my Zeiss Standard. Because now DIC is inaffordable for my budget, this techi...

Charles, first and second are excellent but... :shock: ...last one is absolutely extraordinary. I never saw a comparable light micrography of striated muscle whith all this detail and clarity in the bands, it's similar to low power TEM images shown in biology textbooks, but alive!:smt038 . This musc...

Very impressive!

The stained oval particle outside neutrophils, is a fungal cell?

Daniel wrote: "Since years I use a special camera connector sold to me by the manufacurer to attach the camera to the microscope." Is the adapter branded Leitz? Not branded, but it was sold to me by Leica who also takes care of Leitz microscopes... Can it be dismounted?. If affirmative, doses it co...

Joaquim,
This setup doesnt seem more compact or easy to use than your former tests coupling the infinite objective on a 200mm tele lens. Are your results better whith this aproach?

Daniel wrote: "Since years I use a special camera connector sold to me by the manufacurer to attach the camera to the microscope." Is the adapter branded Leitz? Can it be dismounted?. If affirmative, doses it contain an eyepiece inside? Leitz objectives need correction of both CA and flatness of fie...

Wow, you have some good equipement!
Thank you for the information about StackShot.
Apart of some stacking artifacts, the pictures look excellent, but the last one whith its long anthenae cant't be Drosophila

Paul, very good comparation.
I need to test the JML against my old Oly 20mm f3.5 (single coated) but I think I already know the answer: the JML may be the winner.

When we will be able to buy it for $10 like the JML lens? (lots of emoticons)

Thanks all for your comments. that last one would make a nice poster. Good point!, but it's already at 100%, it will be a very little one :) I guess the objective does not claim to be apochromatic (and not all lenses which do claim it are, in fact, apochromatic). Since axial CA is an artifact of OOF...

Just received and preliminary tested a Nikon CF Plan 10X 0.30 infinity/0 WD 16.5. Following the Rogelio's setup, I focused it to the camera sensor whith an Olympus OM 200mm f4. The objective was mounted in a Zeiss Standard microscope whithout head and the camera and lens vertically suspended over it...