Search found 2653 matches

by NikonUser
Thu Apr 08, 2021 2:00 pm
Forum: Equipment Discussions
Topic: Nikon MPlan 60x ELWD NA 0.70 objective
Replies: 0
Views: 61

Nikon MPlan 60x ELWD NA 0.70 objective

I used this objective to photograph the shell of a Ostracod, last image: http://www.photomacrography.net/forum/viewtopic.php?f=27&t=43360 The image was poor. Thinking that maybe the poor result was more the nature of the subject than the quality of the lens. The shell was strongly convex. Used the s...
by NikonUser
Wed Apr 07, 2021 7:44 am
Forum: Photography Through the Microscope
Topic: Polarized midge larvae
Replies: 5
Views: 122

Re: Polarized mosquito (or midge) larvae

Excellent image; it's a Chironomid (midge)
by NikonUser
Wed Apr 07, 2021 6:33 am
Forum: Technical and Studio Photography -- Macro and Close-up
Topic: Seed Shrimp shell details
Replies: 3
Views: 162

Re: Seed Shrimp shell details

I photographed part of the shell with a Nikon 60x ELWD MPlan objective; NA 0.70
Rather poor image quality; not an improvement of the actual pixels from the 20x.
by NikonUser
Wed Apr 07, 2021 3:51 am
Forum: Technical and Studio Photography -- Macro and Close-up
Topic: Patterns in Nature
Replies: 4
Views: 496

Re: Patterns in Nature

This subject:
viewtopic.php?f=27&t=43360

would fit here
by NikonUser
Tue Apr 06, 2021 2:10 pm
Forum: Technical and Studio Photography -- Macro and Close-up
Topic: Seed Shrimp shell details
Replies: 3
Views: 162

Seed Shrimp shell details

micro_pix showed images of Ostracod shells from a marine environment. The shells appear to have many large pores: http://www.photomacrography.net/forum/viewtopic.php?f=14&t=43258&sid=2ac28e4040ae51f0d3444f0a7f41a289 I have shown images of entire animals here: http://www.photomacrography.net/forum/vi...
by NikonUser
Tue Apr 06, 2021 5:58 am
Forum: Photography Through the Microscope
Topic: Spring Sphagnum Squeeze
Replies: 10
Views: 202

Re: Spring Sphagnum Squeeze

Excellent
by NikonUser
Tue Apr 06, 2021 5:57 am
Forum: Photography Through the Microscope
Topic: Copepod carrying eggs
Replies: 2
Views: 92

Re: Copepod carrying eggs

In another post you mentioned testing your polarization setup.
A copepod such as this one of yours is a great test for polarization.
See:
https://www.microbehunter.com/microscop ... id#p101006
by NikonUser
Mon Apr 05, 2021 11:50 am
Forum: Photography Through the Microscope
Topic: When is a clam not a clam?
Replies: 1
Views: 104

When is a clam not a clam?

When it is an Ostracod! These 2-shelled Crustacea, also known as seed-shrimps , were common in a recent FW sample. They range in size, the largest in this sample is 0.60 mm long. Under polarized light the shell can show several different colours. Olympus BH2/BHS, SPlan 10x + 1.25x intermediate tube ...
by NikonUser
Sun Apr 04, 2021 2:17 pm
Forum: Photography Through the Microscope
Topic: Springtail polarized
Replies: 10
Views: 281

Re: Springtail polarized

Yes, I believe it is a full lambda plat'; labeled 530 nm.
By rotating the polarizer I can get various colour backgrounds; the setting that gives the awful purple colour also gives the best definition of the internal musculature.
by NikonUser
Sun Apr 04, 2021 6:15 am
Forum: Photography Through the Microscope
Topic: Springtail polarized
Replies: 10
Views: 281

Re: Springtail polarized

Thanks again. I worked on the Zerene stacked image and got a better image than my original post. However, it was still slightly inferior to your adjusted image. Had most trouble with removing the purple around the springtail's rear end and furculum, desaturation left me with dark pixels between the ...
by NikonUser
Sat Apr 03, 2021 5:07 am
Forum: Photography Through the Microscope
Topic: Springtail polarized
Replies: 10
Views: 281

Re: Springtail polarized

Thanks Lou. Not only did you vastly improve the background but also the musculature became clearer.
As an extra bonus it shows that high NA condensers can be used with low NA objectives, if one is competent in post-processing.
by NikonUser
Fri Apr 02, 2021 1:32 pm
Forum: Photography Through the Microscope
Topic: Springtail polarized
Replies: 10
Views: 281

Re: Springtail polarized

The uneven-lighted background is a result of me using the wrong condenser, i.e., their BH2-NC (not recommended for low NA objectives). The Olympus 4x D Plan has a NA of 0.10 and is meant to be used with their Ultra Low Condenser (ULC), which I have. The ULC gave a very evenly-illuminated background ...
by NikonUser
Fri Apr 02, 2021 8:31 am
Forum: Photography Through the Microscope
Topic: Springtail polarized
Replies: 10
Views: 281

Springtail polarized

extracted this small, body length 1.0 mm, Collembola from moss that had just been exposed below melting snow. In normal light seen as a small off-white beastie, polarized light shows the internal musculature - red, blue, yellow. Note the large yellow dorsal muscle at the rear end which pulls the fur...
by NikonUser
Fri Mar 26, 2021 12:42 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Hand sectioning; plants
Replies: 14
Views: 3530

Re: Hand sectioning; plants

A further, final, comment on hand sectioning plant material; (originally posted on MicrobeHunter). I have been trying several techniques to get cross-sections of plant stems including 'hand' cutting with the double razor blade, using a hand microtome fitted to a bench with the plant held by a foam p...
by NikonUser
Fri Mar 26, 2021 6:16 am
Forum: Technical and Studio Photography -- Macro and Close-up
Topic: Patterns in Nature
Replies: 4
Views: 496

Patterns in Nature

I sampled my local ditch for aquatics to photograph. Lots of leaves from 2020. There was one aspen leaf (Populus sp.) that was likely from the year 2019 as it had been heavily skeletonized.