Search found 2635 matches

by NikonUser
Sun Mar 07, 2021 5:32 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Hand sectioning; plants
Replies: 9
Views: 2414

Re: Hand sectioning; plants

Thanks Sumguy01; it seems that keeping the blades perpendicular to the stem results in a complete section but rather thick. I do sometimes end up with some thin sections but these are never complete 'circles'; so I get maybe 1/2 a section but the inner straight edge is just a few cells thick. Likely...
by NikonUser
Sun Mar 07, 2021 8:22 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Hand sectioning; plants
Replies: 9
Views: 2414

Re: Hand sectioning; plants

My botanical stains finally arrived (from the UK). Here are two sections of a lupine stem using the double-blade method; stained with Safranin-O and Fast Green. Reversed 40mm enlarging lens and Nikon 10x for close up, Nikon D700 Rather thick sections but considering the cost of equipment (= 2 razor ...
by NikonUser
Thu Feb 25, 2021 4:17 am
Forum: Photography Through the Microscope
Topic: First attempt at a thin section with a manual microtome
Replies: 10
Views: 430

Re: First attempt at a thin section with a manual microtome

The double-blade method works well. Will not always give an intact section, will do so for thick sections, but it will give a portion of the section that can be in the region of a few cells deep. Equipment needed is cheap. http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.or...
by NikonUser
Sat Feb 13, 2021 12:51 pm
Forum: Equipment Discussions
Topic: Photo Stacking System
Replies: 4
Views: 625

Re: Photo Stacking System

My BHS came with a rubber cap to fit the fine adjustment knob "increases the sensitivity of the fine focus motion" (optionally available for the BHS).
Looks similar to your nylon one, slightly tapered 'hole'.
by NikonUser
Sat Jan 23, 2021 7:16 am
Forum: Beginners Micro
Topic: First few days of micro
Replies: 11
Views: 1641

subject drifting

Zerene does an incredible job to correct for this. I have had specimens in water that moved considerably between frames.

By checking "Align each frame against first frame separately" in Preferences, all frames line up.
by NikonUser
Fri Jan 22, 2021 9:37 am
Forum: Beginners Micro
Topic: First few days of micro
Replies: 11
Views: 1641

Re: First few days of micro

Your image does seem to be less sharp than I would expect from that lens. I just happened to be photographing a bug head with the same objective, Nikon D7200, 98 frames @ 0.006 mm. 2 diffused flashes, Zerene PMax. Original frames were RAW and processed in Adobe RAW and saved as .tif Small image is t...
by NikonUser
Thu Jan 07, 2021 2:18 pm
Forum: Beginners Micro
Topic: Olympus BH-2 Camera adapter
Replies: 6
Views: 1138

Re: Olympus BH-2 Camera adapter

That's a BHS, a superb scope; I have the same. You need an Olympus NFK projection eyepiece, preferably a 2.5x. Simplest way, and cheapest, is to support the camera (no lens) at 150 mm (I believe that is the correct distance) above the NFK. I have a wooden frame that holds my Nikon DSLR. A cardboard ...
by NikonUser
Sun Nov 22, 2020 6:47 pm
Forum: Technical and Studio Photography -- Macro and Close-up
Topic: Floppy-ear Bunnies
Replies: 7
Views: 2283

Re: Floppy-ear Bunnies

As you likely know, they are tiny. They were collected a few days ago in a Berlese Funnel sample along with quite a few large hairy elongate species. Weather here is cool to cold and obvious wildlife is absent. So, collected some old leaves in odd corners of the garden and ran them through the funne...
by NikonUser
Wed Nov 18, 2020 6:19 pm
Forum: Technical and Studio Photography -- Macro and Close-up
Topic: Floppy-ear Bunnies
Replies: 7
Views: 2283

Re: Floppy-ear Bunnies

Yes, they belong to the 4th Oder in the following classification:

Class Collembola - Springtails and allies

Order Poduromorpha
Order Entomobryomorpha - Elongate-bodied Springtails
Order Neelipleona
Order Symphypleona - Globular Springtails
by NikonUser
Wed Nov 18, 2020 6:06 am
Forum: Technical and Studio Photography -- Macro and Close-up
Topic: Floppy-ear Bunnies
Replies: 7
Views: 2283

Floppy-ear Bunnies

If not, then what ?
Nikon D7200 CFN 10x finite, Zerene stack
by NikonUser
Tue Nov 17, 2020 6:47 pm
Forum: Nature Photography -- Macro and Close-up
Topic: An assorted mix, including a Mystery Critter Quiz
Replies: 3
Views: 1469

Re: An assorted mix, including a Mystery Critter Quiz

What - no prize for correctly guessing!
How about Scorpions of the Pseudo type?
by NikonUser
Tue Nov 03, 2020 4:27 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Hand sectioning; plants
Replies: 9
Views: 2414

Re: Hand sectioning; plants

The only blades my pharmacy had were Wilkinson Sword, made in Germany. I bought a pack, of 10, $14.00 !!
These are the ones I used.
Still waiting for my stains, from the UK.
by NikonUser
Thu Oct 29, 2020 6:38 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Hand sectioning; plants
Replies: 9
Views: 2414

Hand sectioning; plants

In this month's Micscape Magazine there is an interesting technique: http://www.microscopy-uk.org.uk/mag/indexmag.html I have tried to hand cut sections using several methods, none worked! This method works at almost no cost. Here is a section of a Hydrangea flower stem. I have only zoological stain...
by NikonUser
Thu Oct 01, 2020 5:28 pm
Forum: Nature Photography -- Macro and Close-up
Topic: Dream Prince
Replies: 2
Views: 1883

Re: Dream Prince

Rana temporaria
by NikonUser
Wed Sep 23, 2020 6:05 am
Forum: General Discussion Forum and Community Announcements
Topic: Searching for image stacks for AI training
Replies: 4
Views: 1774

Re: Searching for image stacks for AI training

I still have the 13 images that made this final image; any use?